GENEALOGY-DNA-L ArchivesArchiver > GENEALOGY-DNA > 2005-12 > 1134867874
Subject: PubMed abstract: comprehensive map of Y STRs
Date: Sat, 17 Dec 2005 20:04:34 EST
Leg Med (Tokyo). 2005 Dec 6; [Epub ahead of print]
Comprehensive annotated STR physical map of the human Y chromosome: Forensic
Hanson EK, Ballantyne J.
Graduate Program in Biomolecular Science, University of Central Florida, P.O.
Box 162366, Orlando, FL, 32816-2366; National Center for Forensic Science,
P.O. Box 162367, Orlando, FL 32816-2367.
A plethora of Y-STR markers from diverse sources have been deposited in
public databases and represent potential candidates for incorporation into the next
generation of Y-STR multiplexes for forensic use. Here, based upon all of the
Y-STR loci that have been deposited in the human genome database (>400), we
have sequentially positioned each one along the Y chromosome using the most
current human genome sequencing data (NCBI Build 35). The information derived
from this work defines the number and relative position of all potentially
forensically relevant Y-STR loci, their location within the physical linkage map of
the Y chromosome and their relationship to structural genes. We conclude that
there exists at present at least 417 separate Y-STR markers available for
potential forensic use, although many of these will be found to be unsuitable for
other reasons. However, from this data, we were able to identify 28 pairs of
duplicated loci that were given separate DYS designations and four pairs of
loci with overlapping flanking regions. Removing one locus from each set of
duplicates reduced the number of potentially useful loci from 417 to 389. The
derived information should be useful for workers who are designing novel Y-STR
multiplexes to ensure the presence of non-synonymous loci and, if so desired, to
avoid loci that lie within structural genes. It may also be useful for forensic
casework practitioners (or molecular anthropologists) to aid in
distinguishing between chromosomal rearrangements (such as duplications and deletions) and
bona fide DNA admixtures or null alleles caused by primer binding site
mutations. We illustrate the practical usefulness of the chromosomal positioning data
in the design of eight multiplex systems using 94 Y-STR loci.
PMID: 16337821 [PubMed - as supplied by publisher]