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From: steven perkins <>
Subject: [DNA] Article: Nuclear DNA Typing From Ancient Teeth.
Date: Mon, 15 Nov 2010 22:53:05 -0500


For those interested in aDNA analysis:
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http://journals.lww.com/amjforensicmedicine/Abstract/publishahead/Nuclear_DNA_Typing_From_Ancient_Teeth.99802.aspx

Am J Forensic Med Pathol. 2010 Nov 5. [Epub ahead of print]

Nuclear DNA Typing From Ancient Teeth.

Sosa C, Baeta M, Núñez C, Casalod Y, Luna A, Martínez-Jarreta B.

>From the *Department of Forensic Medicine, Faculty of Medicine,
University of Zaragoza, Zaragoza; and †Department of Forensic
Medicine, Faculty of Medicine, University of Murcia, Campus del
Espinardo, Murcia, Spain.
Abstract

Because of the adverse effects that diagenesis exert on ancient
skeletal remains, DNA from these samples is often compromised to the
point where genetic typing can be challenging. Nevertheless, robust
and reliable methods are currently available to allow successful
genotyping of ancient specimens. Here we report nuclear DNA-based
methods and typing strategies used to analyze 2 human skeletons from a
medieval burial. Reliable DNA nuclear profiles were obtained from
teeth, whereas mitochondrial DNA analyses in bones were inconclusive.
A complete nuclear mini short tandem repeat profile was obtained from
a well-preserved premolar, but only a partial one from the femur.
Increasing the sensitivity of the polymerase chain reaction system
allowed a full profile from the latter, but the presence of artifacts
reinforced the idea that the interpretation of this kind of analysis
must be performed with caution. The results presented here also
indicate that DNA from dental pieces can be better preserved than from
bones, even in the case of well-preserved long bones with thick
cortical tissue such as the femurs, and have a better chance of
successful genetic typing, probably because of the high degree of
protection conferred to the DNA by the enamel.

PMID: 21063198 [PubMed - as supplied by publisher]

--
Steven C. Perkins      
http://stevencperkins.com/
Online Journal of Genetics and Genealogy
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http://scpgen.blogspot.com/


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